Determination of the relative amounts of Gag and Pol proteins in foamy virus particles.

Retrovirology. 2005 Jul 8;2:44.

Cartellieri M, Rudolph W, Herchenröder O, Lindemann D, Rethwilm A.

We determined the relative ratios of Gag and Pol molecules in highly purified virions of spumaretroviruses or foamy viruses (FVs) using monoclonal antibodies and bacterially expressed reference proteins. We found that the cleaved p68Gag moiety dominates in infectious FVs. Furthermore, approximate mean ratios in FV are 16:1 (pr71Gag plus p68Gag:p85RT),12:1 (p68Gag:p85RT), and 10:1 (pr71Gag plus p68Gag:p40IN). Thus, the results indicate that FVs have found a way to incorporate approximately as much Pol protein into their capsids as orthoretroviruses, despite a completely different Pol expression strategy.

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The RET proto-oncogene: A valuable target for molecular cancer therapy.

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N-terminal Gag domain required for foamy virus particle assembly and export.

Determination of the relative amounts of Gag and Pol proteins in foamy virus particles.

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